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a – c SoTILT3D combines a <t>microfluidic</t> chip that has a nanoprinted insert with a metalized side wall with a single-objective tilted light sheet (LS) and is compatible with epi- and transmission illumination. The geometry and size of the insert and the microfluidic chip can be flexibly adjusted for different targets. Schematics not to scale. d Scanning electron micrograph of the microfluidic chip insert design shown in ( b ). Scale bar 50 μm. Insert geometry was confirmed with conventional optical microscopy for each microfluidic chip used in this work. e The thin end of the LS imaged in a fluorescent solution. Scale bar 10 μm. The colorbar shows normalized intensity. f Fluorescent beads imaged with the 2 μm axial range double-helix point spread function (DH-PSF) at the indicated positions. Scale bar 2 μm. The colorbar shows normalized intensity. Fluorescent beads were imaged with the 2 μm DH-PSF for all 3D single-molecule data included in this work, with consistent performance across acquisitions.
Microfluidic Chip Stagetop Incubation Chamber P 736 Zr1s/Zr2s, supplied by Okolab USA Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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two-chamber microfluidic chip sd150  (Xona Microfluidics)
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a – c SoTILT3D combines a <t>microfluidic</t> chip that has a nanoprinted insert with a metalized side wall with a single-objective tilted light sheet (LS) and is compatible with epi- and transmission illumination. The geometry and size of the insert and the microfluidic chip can be flexibly adjusted for different targets. Schematics not to scale. d Scanning electron micrograph of the microfluidic chip insert design shown in ( b ). Scale bar 50 μm. Insert geometry was confirmed with conventional optical microscopy for each microfluidic chip used in this work. e The thin end of the LS imaged in a fluorescent solution. Scale bar 10 μm. The colorbar shows normalized intensity. f Fluorescent beads imaged with the 2 μm axial range double-helix point spread function (DH-PSF) at the indicated positions. Scale bar 2 μm. The colorbar shows normalized intensity. Fluorescent beads were imaged with the 2 μm DH-PSF for all 3D single-molecule data included in this work, with consistent performance across acquisitions.
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perfusion chamber of an ibidi microfluidic chip μ-slide vi 0.5 glass bottom  (ibidi GmbH)
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a – c SoTILT3D combines a <t>microfluidic</t> chip that has a nanoprinted insert with a metalized side wall with a single-objective tilted light sheet (LS) and is compatible with epi- and transmission illumination. The geometry and size of the insert and the microfluidic chip can be flexibly adjusted for different targets. Schematics not to scale. d Scanning electron micrograph of the microfluidic chip insert design shown in ( b ). Scale bar 50 μm. Insert geometry was confirmed with conventional optical microscopy for each microfluidic chip used in this work. e The thin end of the LS imaged in a fluorescent solution. Scale bar 10 μm. The colorbar shows normalized intensity. f Fluorescent beads imaged with the 2 μm axial range double-helix point spread function (DH-PSF) at the indicated positions. Scale bar 2 μm. The colorbar shows normalized intensity. Fluorescent beads were imaged with the 2 μm DH-PSF for all 3D single-molecule data included in this work, with consistent performance across acquisitions.
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perfusion chamber of an ibidi microfluidic chip  (ibidi GmbH)
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a – c SoTILT3D combines a <t>microfluidic</t> chip that has a nanoprinted insert with a metalized side wall with a single-objective tilted light sheet (LS) and is compatible with epi- and transmission illumination. The geometry and size of the insert and the microfluidic chip can be flexibly adjusted for different targets. Schematics not to scale. d Scanning electron micrograph of the microfluidic chip insert design shown in ( b ). Scale bar 50 μm. Insert geometry was confirmed with conventional optical microscopy for each microfluidic chip used in this work. e The thin end of the LS imaged in a fluorescent solution. Scale bar 10 μm. The colorbar shows normalized intensity. f Fluorescent beads imaged with the 2 μm axial range double-helix point spread function (DH-PSF) at the indicated positions. Scale bar 2 μm. The colorbar shows normalized intensity. Fluorescent beads were imaged with the 2 μm DH-PSF for all 3D single-molecule data included in this work, with consistent performance across acquisitions.
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zellsafetm cells chipcytometry microfluidic chambers (chips)  (Zellkraftwerk gmbh)
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Zellsafetm Cells Chipcytometry Microfluidic Chambers (Chips), supplied by Zellkraftwerk gmbh, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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microfluidic chamber array chips for single cell capture  (Momentive Performance Materials)
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a – c SoTILT3D combines a <t>microfluidic</t> chip that has a nanoprinted insert with a metalized side wall with a single-objective tilted light sheet (LS) and is compatible with epi- and transmission illumination. The geometry and size of the insert and the microfluidic chip can be flexibly adjusted for different targets. Schematics not to scale. d Scanning electron micrograph of the microfluidic chip insert design shown in ( b ). Scale bar 50 μm. Insert geometry was confirmed with conventional optical microscopy for each microfluidic chip used in this work. e The thin end of the LS imaged in a fluorescent solution. Scale bar 10 μm. The colorbar shows normalized intensity. f Fluorescent beads imaged with the 2 μm axial range double-helix point spread function (DH-PSF) at the indicated positions. Scale bar 2 μm. The colorbar shows normalized intensity. Fluorescent beads were imaged with the 2 μm DH-PSF for all 3D single-molecule data included in this work, with consistent performance across acquisitions.
Microfluidic Chamber Array Chips For Single Cell Capture, supplied by Momentive Performance Materials, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lab-on-chip microfluidics chamber  (Multigate Medical Products Pty Ltd)
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Multigate Medical Products Pty Ltd lab-on-chip microfluidics chamber
a – c SoTILT3D combines a <t>microfluidic</t> chip that has a nanoprinted insert with a metalized side wall with a single-objective tilted light sheet (LS) and is compatible with epi- and transmission illumination. The geometry and size of the insert and the microfluidic chip can be flexibly adjusted for different targets. Schematics not to scale. d Scanning electron micrograph of the microfluidic chip insert design shown in ( b ). Scale bar 50 μm. Insert geometry was confirmed with conventional optical microscopy for each microfluidic chip used in this work. e The thin end of the LS imaged in a fluorescent solution. Scale bar 10 μm. The colorbar shows normalized intensity. f Fluorescent beads imaged with the 2 μm axial range double-helix point spread function (DH-PSF) at the indicated positions. Scale bar 2 μm. The colorbar shows normalized intensity. Fluorescent beads were imaged with the 2 μm DH-PSF for all 3D single-molecule data included in this work, with consistent performance across acquisitions.
Lab On Chip Microfluidics Chamber, supplied by Multigate Medical Products Pty Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a – c SoTILT3D combines a microfluidic chip that has a nanoprinted insert with a metalized side wall with a single-objective tilted light sheet (LS) and is compatible with epi- and transmission illumination. The geometry and size of the insert and the microfluidic chip can be flexibly adjusted for different targets. Schematics not to scale. d Scanning electron micrograph of the microfluidic chip insert design shown in ( b ). Scale bar 50 μm. Insert geometry was confirmed with conventional optical microscopy for each microfluidic chip used in this work. e The thin end of the LS imaged in a fluorescent solution. Scale bar 10 μm. The colorbar shows normalized intensity. f Fluorescent beads imaged with the 2 μm axial range double-helix point spread function (DH-PSF) at the indicated positions. Scale bar 2 μm. The colorbar shows normalized intensity. Fluorescent beads were imaged with the 2 μm DH-PSF for all 3D single-molecule data included in this work, with consistent performance across acquisitions.

Journal: Nature Communications

Article Title: Whole-cell multi-target single-molecule super-resolution imaging in 3D with microfluidics and a single-objective tilted light sheet

doi: 10.1038/s41467-024-54609-z

Figure Lengend Snippet: a – c SoTILT3D combines a microfluidic chip that has a nanoprinted insert with a metalized side wall with a single-objective tilted light sheet (LS) and is compatible with epi- and transmission illumination. The geometry and size of the insert and the microfluidic chip can be flexibly adjusted for different targets. Schematics not to scale. d Scanning electron micrograph of the microfluidic chip insert design shown in ( b ). Scale bar 50 μm. Insert geometry was confirmed with conventional optical microscopy for each microfluidic chip used in this work. e The thin end of the LS imaged in a fluorescent solution. Scale bar 10 μm. The colorbar shows normalized intensity. f Fluorescent beads imaged with the 2 μm axial range double-helix point spread function (DH-PSF) at the indicated positions. Scale bar 2 μm. The colorbar shows normalized intensity. Fluorescent beads were imaged with the 2 μm DH-PSF for all 3D single-molecule data included in this work, with consistent performance across acquisitions.

Article Snippet: The microfluidic chip was mounted in a stagetop incubation chamber (P-736-ZR1S/ZR2S, OkoLab) compatible with an xy translation stage (OPH-XYS-O, Physik Instrumente) and a fine adjustment xyz piezoelectric translation stage (OPH-PINANO-XYZ, Physik Instrumente).

Techniques: Transmission Assay, Microscopy